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KMID : 0358219960230020121
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Korean Journal of Fertility and Sterility
1996 Volume.23 No. 2 p.121 ~ p.128
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Chromosomal Abnormality According to the Morphology of Human Preimplanation Embryos
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김해정
윤현수/이동률/이정현/조정현/노성일
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Abstract
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Preimplantation genetic diagnosis by fluorescence in situ hybridization (FISH) allows the numeric and structural analysis of chromosomes in human embrgos. Many embryos obtained by in vitro fertilization (IVF) either fail to implant or abort soon
after
implantation. It was suggested that the lost embryos have high rate of chromosomal abnormalities and morphological abnormalities (dysmorphism). The purpose of this study was to analyze the relationship between morphology and numerical chromosomal
abnormalities in human preimplantation embryos using multiple-probe FISH. The hybridization targets for the Nos 8, 15, 18, X and Y probes were α-satellite repeat clusters in the centromeric region and satellite Ⅲ DNA at Yqh, respectively. The
efficiency of isolation and fixation of blastomeres was 80% (86/107) and 89.5% (77/86) of blastomeres showed hybridization signals. Morphologically normal monospermic embryos had also chromosomal abnormalities (60%, p<0.05). All of multinucleated
blastomeres (6.6) were found chromosomal abnormalities. The present study showed that embryos derived from normally fertilized zygotes had also high rate of chromosomal aneuploidies and that embryonic morphological abnormalities were correlated
with
chromosomal aneuploidies. Therefore, it is suggested that PGD will increase the success rate of implantation by eliminating abnormal embryos with aneuploidy from the transfer procedure in IVF-ET.
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